1. What is Peptide Reconstitution?

Peptide reconstitution is the process of dissolving lyophilized (freeze-dried) peptides in a suitable solvent to prepare them for experimental or therapeutic use. The concentration of the final solution is critical for accurate dosing.

2. How Does the Calculator Work?

The calculator uses the basic concentration formula:

Where:

• \( C \) — Concentration in milligrams per milliliter (mg/mL)
• \( mg \) — Mass of peptide in milligrams
• \( mL \) — Volume of solvent in milliliters

Explanation: This simple formula calculates the concentration of peptide solution by dividing the mass of peptide by the volume of solvent used.

3. Importance of Accurate Concentration

Details: Precise peptide concentration is essential for:

• Accurate experimental dosing
• Reproducible research results
• Proper therapeutic administration
• Consistency between batches

4. Using the Calculator

Tips:

• Use precise measurements of peptide mass (preferably with analytical balance)
• Measure solvent volume accurately (use graduated cylinder or pipette)
• Ensure complete dissolution of peptide before use
• Store reconstituted peptides properly (often at -20°C or lower)

5. Frequently Asked Questions (FAQ)

Q1: What solvent should I use for peptide reconstitution?
A: This depends on the peptide's properties. Common solvents include sterile water, bacteriostatic water, acetic acid, or PBS. Consult the peptide datasheet.

Q2: How long can I store reconstituted peptides?
A: Storage time varies but typically ranges from 1 week at 4°C to several months at -20°C or -80°C. Avoid repeated freeze-thaw cycles.

Q3: What if my peptide doesn't dissolve completely?
A: Try gentle warming, sonication, or adding small amounts of organic solvent (like DMSO). Check peptide solubility characteristics first.

Q4: How do I calculate dosing from concentration?
A: Use the formula: Volume to inject (mL) = Desired dose (mg) / Concentration (mg/mL)

Q5: Why is my calculated concentration different from expected?
A: Possible reasons include inaccurate weighing, solvent evaporation, incomplete dissolution, or peptide degradation.