1. What is Peptide Reconstitution?

Peptide reconstitution is the process of dissolving lyophilized (freeze-dried) peptides in a suitable solvent to achieve a desired concentration for experimental or therapeutic use.

2. How Does the Calculator Work?

The calculator uses the basic concentration formula:

Where:

• \( mass \) — Mass of peptide (in mg or μg)
• \( volume \) — Volume of solvent (in mL)
• \( Concentration \) — Resulting concentration (in mg/mL or μg/mL)

Explanation: The calculator automatically handles unit conversions between mg and μg for both input and output.

3. Importance of Accurate Reconstitution

Details: Precise peptide reconstitution is critical for experimental reproducibility, proper dosing in therapeutic applications, and maintaining peptide stability.

4. Using the Calculator

Tips:

• Use the mass as listed on your peptide vial (typically in mg)
• Measure solvent volume precisely
• Select appropriate units for your application (research often uses μg/mL)
• All values must be positive numbers

5. Frequently Asked Questions (FAQ)

Q1: What solvent should I use for peptide reconstitution?
A: This depends on the peptide. Common solvents include sterile water, PBS, or acetic acid solutions. Consult your peptide's documentation.

Q2: How should I store reconstituted peptides?
A: Most peptides should be aliquoted and stored at -20°C or -80°C to prevent degradation.

Q3: What concentration should I use for my experiment?
A: This depends on your specific protocol. Typical working concentrations range from 0.1-100 μg/mL for most applications.

Q4: How long do reconstituted peptides remain stable?
A: Stability varies by peptide, but most remain stable for weeks when properly stored. Avoid repeated freeze-thaw cycles.

Q5: Can I use this calculator for proteins?
A: Yes, the same calculation applies to protein reconstitution, though protein concentrations are typically expressed in mg/mL.