1. What is the Peptide Calculator?

The Peptide Calculator helps researchers determine the amount of peptide needed for experiments based on desired concentration, volume, molecular weight, and purity. It's particularly useful for Bachem peptides and other research-grade peptides.

2. How Does the Calculator Work?

The calculator uses the following formula:

Where:

• Concentration — Desired final concentration in millimolar (mM)
• Volume — Total volume needed in milliliters (mL)
• MW — Molecular weight of the peptide in grams per mole (g/mol)
• Purity — Peptide purity percentage (typically 70-99%)

3. Importance of Accurate Peptide Calculations

Details: Precise peptide calculations are essential for reproducible experiments, proper dosing in biological assays, and cost-effective use of expensive research peptides.

4. Using the Calculator

Tips: Enter the desired concentration in mM, total volume in mL, molecular weight in g/mol, and purity percentage. The calculator will determine how much peptide (in mg) you need to weigh out.

5. Frequently Asked Questions (FAQ)

Q1: Where can I find the molecular weight of my peptide?
A: The MW is typically provided on the certificate of analysis from Bachem or other suppliers. You can also calculate it from the amino acid sequence.

Q2: Should I account for peptide counterions in the MW?
A: Yes, if your peptide comes as a salt (e.g., acetate, TFA), include the counterion mass in your MW calculation.

Q3: How does purity affect the calculation?
A: Lower purity means you need to weigh out more peptide to get the same amount of active material. The calculator automatically adjusts for purity.

Q4: Can I use this for non-peptide compounds?
A: Yes, the same calculation works for any compound where you know the molecular weight.

Q5: What if my peptide is in nmol rather than mg?
A: To convert from mg to nmol: nmol = (mg × 1000) / MW