1. What is the Oligo Resuspension Calculator?

The Oligo Resuspension Calculator determines the volume of liquid needed to dissolve oligonucleotides to achieve a desired concentration. This is essential for molecular biology experiments involving DNA or RNA oligos.

2. How Does the Calculator Work?

The calculator uses the formula:

Where:

• \( V \) — Volume in microliters (μL)
• \( nmol \) — Amount of oligonucleotide in nanomoles
• \( C \) — Desired concentration in micromolar (μM)

Explanation: The formula converts nmol to pmol (×1000) and divides by the desired concentration to get the volume in μL needed for resuspension.

3. Importance of Accurate Resuspension

Details: Proper resuspension volume ensures correct oligo concentration for downstream applications like PCR, sequencing, or hybridization. Incorrect concentrations can lead to failed experiments or inconsistent results.

4. Using the Calculator

Tips: Enter the amount of oligo in nmol (usually provided by the manufacturer) and your desired working concentration in μM. Both values must be positive numbers.

5. Frequently Asked Questions (FAQ)

Q1: Why multiply by 1000 in the formula?
A: The multiplication converts nmol to pmol (1 nmol = 1000 pmol) to match the μM concentration unit (1 μM = 1 pmol/μL).

Q2: What's a typical oligo concentration for resuspension?
A: Common working concentrations range from 10-100 μM, with 100 μM being standard for stock solutions.

Q3: Should I use molecular grade water for resuspension?
A: Yes, nuclease-free molecular grade water or TE buffer is recommended to prevent oligo degradation.

Q4: How should I mix the oligo after resuspension?
A: Vortex briefly and spin down, or mix by pipetting up and down. Avoid excessive vortexing which can shear DNA.

Q5: How should I store resuspended oligos?
A: Store at -20°C for long-term storage. For frequent use, aliquoting is recommended to avoid freeze-thaw cycles.