1. What is Oligo Molarity?

Oligo molarity refers to the concentration of oligonucleotides in solution, expressed in moles per liter (mol/L). It's essential for molecular biology experiments to ensure proper stoichiometry of reactions.

2. How Does the Calculator Work?

The calculator uses the molarity equation:

Where:

• \( M \) — Molarity (mol/L)
• \( mass \) — Mass of oligo (g)
• \( MW \) — Molecular weight (g/mol)
• \( V \) — Volume of solution (L)

Explanation: The equation calculates how many moles of oligo are present per liter of solution, based on the mass of oligo used, its molecular weight, and the total volume of solution.

3. Importance of Molarity Calculation

Details: Accurate molarity calculation is crucial for PCR, sequencing, and other molecular biology techniques where precise oligo concentrations are required for optimal results.

4. Using the Calculator

Tips: Enter mass in grams, molecular weight in g/mol, and volume in liters. All values must be positive numbers.

5. Frequently Asked Questions (FAQ)

Q1: How do I determine the molecular weight of my oligo?
A: Most oligo synthesis providers list the molecular weight. You can also calculate it using online tools based on the sequence.

Q2: Can I use this for other solutes besides oligos?
A: Yes, this calculator works for any solute where you know the mass, molecular weight, and solution volume.

Q3: What if my volume is in mL instead of L?
A: Convert mL to L by dividing by 1000 (e.g., 100 mL = 0.1 L) before entering the value.

Q4: How precise should my measurements be?
A: For most applications, measuring mass to 0.1 mg and volume to 0.1 mL is sufficient.

Q5: Does temperature affect the calculation?
A: The calculation itself is temperature-independent, but solution volume can change slightly with temperature.