1. What is DNA Molarity?

DNA molarity refers to the concentration of DNA in a solution expressed in moles per liter (mol/L). It's calculated by dividing the mass concentration (g/L) by the molecular weight of the DNA (g/mol).

2. How Does the Calculator Work?

The calculator uses the DNA molarity equation:

Where:

• \( M \) — Molarity (mol/L)
• \( conc \) — DNA concentration (g/L)
• \( MW \) — Molecular weight (g/mol)

Explanation: The equation converts mass concentration to molar concentration by accounting for the molecular weight of the DNA.

3. Importance of DNA Molarity Calculation

Details: Knowing the molar concentration of DNA is essential for molecular biology experiments like PCR, sequencing, and cloning where precise amounts of DNA are required.

4. Using the Calculator

Tips: Enter DNA concentration in g/L and molecular weight in g/mol. Both values must be positive numbers.

5. Frequently Asked Questions (FAQ)

Q1: How do I determine the molecular weight of my DNA?
A: For double-stranded DNA, multiply the number of base pairs by 650 g/mol (average MW of one bp). For single-stranded DNA, use 330 g/mol per nucleotide.

Q2: What are typical DNA concentrations used in experiments?
A: Common working concentrations range from 1-100 ng/μL (0.001-0.1 g/L) for most applications.

Q3: Can I use ng/μL instead of g/L?
A: Yes, but you'll need to convert units (1 ng/μL = 1 g/L) before calculation.

Q4: Does this work for RNA as well?
A: Yes, the same equation applies for RNA molarity calculations.

Q5: How accurate is this calculation?
A: The calculation is mathematically precise, but accuracy depends on your concentration measurements and MW estimation.