1. What is Cell Dilution Calculation?

The cell dilution calculation determines the final concentration of cells after dilution using the formula C2 = C1 × (V1/V2). This is essential in cell culture, microbiology, and laboratory experiments where precise cell concentrations are required.

2. How Does the Calculator Work?

The calculator uses the cell dilution formula:

Where:

• \( C_1 \) — Initial cell concentration (cells/mL)
• \( V_1 \) — Initial volume (mL)
• \( V_2 \) — Final volume after dilution (mL)
• \( C_2 \) — Final cell concentration (cells/mL)

Explanation: The formula maintains the total number of cells constant while changing their concentration through dilution.

3. Importance of Cell Concentration

Details: Accurate cell concentration is crucial for experiments, drug testing, and maintaining consistent cell cultures. Proper dilution ensures optimal cell density for growth and reproducibility.

4. Using the Calculator

Tips: Enter initial concentration in cells/mL, initial volume in mL, and final volume in mL. All values must be positive numbers. The calculator will compute the final concentration after dilution.

5. Frequently Asked Questions (FAQ)

Q1: What if I know C2 and need to find V2?
A: Rearrange the formula: V2 = (C1 × V1)/C2. This tells you how much diluent to add to reach your target concentration.

Q2: How accurate is this calculation?
A: The calculation is mathematically precise, but actual results depend on accurate measurement of volumes and thorough mixing.

Q3: Can this be used for serial dilutions?
A: Yes, apply the formula sequentially for each dilution step in a series.

Q4: Does this account for cell loss during pipetting?
A: No, the calculation assumes perfect transfer. For critical applications, account for potential pipetting errors.

Q5: What's the maximum dilution factor possible?
A: Practically limited by the ability to detect cells in very dilute solutions. Each field has different practical limits.