1 To 100 Dilution Formula:
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A 1 to 100 dilution means one part of the original solution is mixed with 99 parts of diluent, resulting in a total dilution factor of 100. This reduces the concentration of the original solution by a factor of 100.
The calculator uses the simple dilution formula:
Where:
Explanation: The formula shows that the final concentration is simply the initial concentration divided by the dilution factor (100 in this case).
Details: Accurate dilution calculations are essential in chemistry, biology, and medical laboratories for preparing solutions of desired concentrations from stock solutions.
Tips: Enter the initial concentration in any consistent units (e.g., mol/L, mg/mL). The result will be in the same units. The initial concentration must be greater than zero.
Q1: How do I prepare a 1:100 dilution in practice?
A: Mix 1 mL of stock solution with 99 mL of diluent, or use proportional volumes (e.g., 10 mL + 990 mL).
Q2: Can I use this for serial dilutions?
A: This calculates a single 1:100 dilution. For serial dilutions, you would need to apply the calculation multiple times.
Q3: Does the type of diluent matter?
A: The calculation is independent of diluent type, but in practice you must choose an appropriate solvent that won't react with your solute.
Q4: How precise should my measurements be?
A: Precision depends on your application. Analytical work typically requires volumetric glassware, while rough estimates may use graduated cylinders.
Q5: Can I use different units?
A: Yes, as long as you maintain consistent units for both initial and final concentrations (e.g., mg/mL to mg/mL).