1. What is a 1:4 Dilution?

A 1:4 dilution means one part of the original solution is mixed with three parts of diluent, resulting in a total volume that is four times the original volume. This reduces the concentration of the solute to one quarter of its original value.

2. How Does the Calculator Work?

The calculator uses the simple dilution formula:

Where:

• \( C_1 \) — Initial concentration (mol/L)
• \( C_2 \) — Final concentration after 1:4 dilution (mol/L)

Explanation: The formula accounts for the dilution factor of 4, which is the ratio of the final volume to the initial volume.

3. Importance of Dilution Calculations

Details: Accurate dilution calculations are essential in laboratory work, medical testing, and pharmaceutical preparations to achieve desired concentrations for experiments or treatments.

4. Using the Calculator

Tips: Enter the initial concentration in mol/L. The calculator will automatically compute the final concentration after a 1:4 dilution.

5. Frequently Asked Questions (FAQ)

Q1: What exactly does 1:4 dilution mean?
A: It means 1 part original solution + 3 parts diluent = 4 parts total, resulting in a solution that is one quarter the original concentration.

Q2: How do I prepare a 1:4 dilution in practice?
A: For example, mix 1 mL of stock solution with 3 mL of diluent (water, buffer, etc.) to make 4 mL total volume.

Q3: Can I use this for any concentration units?
A: Yes, as long as you're consistent. The units of C₂ will be the same as C₁ (mol/L, mg/mL, %, etc.).

Q4: What if I need a different dilution factor?
A: The general formula is C₂ = C₁/DF where DF is dilution factor. For 1:5 dilution, DF=5; for 1:10, DF=10, etc.

Q5: Does this account for volume changes during mixing?
A: This assumes ideal behavior where volumes are additive. For very concentrated solutions, actual volume may differ slightly.